137 Recovery of quail spermatogenesis by donor spermatogonia transplantation
| Autor: | N. A. Zinovieva, E. K. Tomgorova, Anastasia N Vetokh, N. A. Volkova, L. A. Volkova |
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| Rok vydání: | 2020 |
| Předmět: |
endocrine system
education.field_of_study urogenital system Population Reproductive technology Biology Sertoli cell Sperm Quail Transplantation Andrology Endocrinology medicine.anatomical_structure Reproductive Medicine biology.animal Genetics medicine Animal Science and Zoology education Molecular Biology Spermatogenesis Germ cell Developmental Biology Biotechnology |
| Zdroj: | Reproduction, Fertility and Development. 32:195 |
| ISSN: | 1031-3613 |
| DOI: | 10.1071/rdv32n2ab137 |
| Popis: | Spermatogonia, the stem cell precursors of male germ cells, are used as convenient biological material for the preservation of genetic resources (cryobanks) and the introduction of recombinant DNA (transgenesis). Donor spermatogonia subsequently differentiate into mature germ cells (spermatozoa), which are used to produce offspring. Our laboratory is investigating methods to improve the efficiency of spermatogonial germ cell transplantation in quail. The objectives of this study were to (1) determine the optimal age for spermatogonia isolation from the testes of donor male quail and (2) identify the most appropriate concentration of busulfan for treatment of recipient quail testes. Statistical analysis was performed using SPSS ver. 15.0 (IBM Corp.; analysis of variance test). In order to determine the optimal age for spermatogonia isolation, testes from male quail at 1-4 weeks of age were isolated and histological studies were performed on a population of spermatogenic cells in the seminiferous tubules. Histological studies of quail testes isolated at different ages showed that the optimal age for obtaining a culture of spermatogonia is a period from 1-2 weeks of age. During this period, spermatogenic cells were represented mainly by spermatogonia (P |
| Databáze: | OpenAIRE |
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