Avian Mycoplasma Identification Using Polymerase Chain Reaction Amplicon and Restriction Fragment Length Polymorphism Analysis
| Autor: | Lauerman Lh, Chilina Ar, Johansen D, Closser Ja |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Genetics
General Immunology and Microbiology Mycoplasma Biology Amplicon medicine.disease_cause Molecular biology law.invention Terminal restriction fragment length polymorphism Restriction enzyme Food Animals law Cleaved amplified polymorphic sequence medicine Animal Science and Zoology Restriction fragment length polymorphism Nested polymerase chain reaction Polymerase chain reaction |
| Zdroj: | Avian Diseases. 39:804 |
| ISSN: | 0005-2086 |
| DOI: | 10.2307/1592417 |
| Popis: | A general mycoplasma polymerase chain reaction (PCR) was used to generate amplicon (DNA amplification product) from nine avian mycoplasma species. The PCR amplicons were reacted with 24 restriction enzymes, and the electrophoretic patterns of restriction fragment length polymorphism (RFLP) were evaluated for differences between species of mycoplasms. Four (DraI, MseI, RsaI, Tsp5091) of the 24 restriction enzymes cut the PCR amplicon of all nine mycoplasma species. The nine avian mycoplasma species could be distinctly differentiated using the RFLP analysis of the PCR amplicon. |
| Databáze: | OpenAIRE |
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