Isolation and characteristics of a short rod-shaped mutant ofPseudomonas aeruginosa

Autor:	Teruo Tanino, Chieko Hanehara, Naomasa Gotoh
Rok vydání:	1984
Předmět:	Cell division Strain (chemistry) Pseudomonas aeruginosa Mutant Clone (cell biology) Mutagenesis (molecular biology technique) Biology biology.organism_classification medicine.disease_cause Microbiology Genetics medicine Doubling time Molecular Biology Pseudomonadaceae
Zdroj:	FEMS Microbiology Letters. 24:345-349
ISSN:	1574-6968 0378-1097
Popis:	Among 40 short rod-shaped mutants of Pseudomonas aeruginosa PAO isolated after nitrosoguanidine mutagenesis, a stable clone designated KG1034 was obtained and studied for its cellular and genetical features. Cells of the parent strain, PAO2302 had a mean cell length of 1.9 μm, whereas that of KG1034 was 1.3 μm. The doubling time of KG1034 was less than that of the parent strain, although both strains elongated at the same rate and exhibited the same relative amounts and pattern of penicillin-binding proteins. These results suggested that the phenotype of KG1034 was due to the initiation of septation at an earlier stage. The new gene responsible for this phenotype was named srs (abbreviation for short rod shape) and mapped by conjugation between cys-54 and puuC.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e82e36ebaac1dad62f76b706710fc030 https://doi.org/10.1111/j.1574-6968.1984.tb01332.x Zobrazit plný text záznamu Plný text