Isolation and purification of biopolymers by affinity chromatography. VI. Preparation and properties of an affinity adsorbent with a polysaccharide spacer for the purification of proteolytic enzymes
| Autor: | V. Kh. Mitina, F. M. Shemyakin, B. A. Klyashchitskii, R. I. Yakubovskaya |
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| Rok vydání: | 1980 |
| Předmět: |
chemistry.chemical_classification
Sorbent Chromatography Kunitz STI protease inhibitor Proteolytic enzymes virus diseases food and beverages Plant Science General Chemistry urologic and male genital diseases Trypsin Polysaccharide female genital diseases and pregnancy complications General Biochemistry Genetics and Molecular Biology carbohydrates (lipids) chemistry.chemical_compound Adsorption chemistry Affinity chromatography medicine Cyanogen bromide medicine.drug |
| Zdroj: | Chemistry of Natural Compounds. 16:405-408 |
| ISSN: | 1573-8388 0009-3130 |
| Popis: | A new affinity sorbent has been synthesized — soybean trypsin inhibitor (STI)-amylopectin-hydrazidosuccinyl-Sepharose — and its properties have been studied in comparison with those of an analogous adsorbent without the spacer STI-Sepharose. The STI-amylopectin-hydrazidosuccinyl-Sepharose adsorbent has been used for the purification of trypsin from porcine pancreas and of callicrein from human blood plasma. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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