The measurement of electron transport system activity in river biofilms
| Autor: | Maurice A. Lock, S.A. Blenkinsopp |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Environmental Engineering
Chromatography Ethanol Chemistry Tetrazolium chloride viruses Ecological Modeling Environmental engineering Biofilm biochemical phenomena metabolism and nutrition Pollution Electron transport chain Propanol chemistry.chemical_compound Iodonitrotetrazolium Methanol Formazan Waste Management and Disposal Water Science and Technology Civil and Structural Engineering |
| Zdroj: | Water Research. 24:441-445 |
| ISSN: | 0043-1354 |
| Popis: | The factors affecting the measurement of electron transport system (ETS) activity in river biofilm through the reduction of 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetrazolium chloride (INT) to iodonitrotetrazolium formazan (INT-formazan) have been studied. Methanol extracts INT-formazan more effectively than either propanol or ethanol. A concentration of 0.02% INT was chosen and samples should be incubated for less than 8 h. ETS activity is optimal at a circumneutral pH. ETS stimulators (NADH, NADPH and succinate) added as a check of the assay produced an increase in INT-formazan indicating that ETS activity was being measured. This assay is quick, easy to use and ideal for field studies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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