| Autor: |
Musa Khaitov, Igor Shilovskiy, Rudolf Valenta, Milena Weber, Korneev Artem, Inna Tulaeva, Pia Gattinger, Marianne van Hage, Gerhard Hofer, Jon Konradsen, Walter Keller, Oluwatoyin Akinfenwa, Alina Poroshina, Nataliya Ilina, Elena Fedenko, Olga G. Elisyutina, Alla Litovkina, Evgenii V. Smolnikov, Alexandra Nikonova, Sergei Rybalkin, Vladimir Aldobaev, Valeriy Smirnov, Nadezhda Shershakova, Olga Petukhova, Dmitry Kudlay, Artem Shatilov, Anastasia Timofeeva, Raffaela Campana, Sevastyan Rabdano, Victor Trukhin, Sergey Udin, Veronica Skvortsova |
| Rok vydání: |
2023 |
| Popis: |
Background: Respiratory birch pollen allergy and associated oral allergy syndrome affect more than 150 million people. IgE cross-sensitization to major birch pollen allergen Bet v 1 and pathogenesis-related (PR10) plant food allergens is responsible for the pollen-food allergy syndrome. Methods: We designed a recombinant protein, AB-PreS, consisting of non-allergenic peptides derived from the IgE binding sites of Bet v 1 and the cross-reactive apple allergen, Mal d 1, fused to the PreS domain of HBV surface protein as immunological carrier. AB-PreS was expressed in E. coli and purified by chromatography. The allergenic activity of AB-PreS was tested using sera and basophils from birch pollen patients allergic. The protective effect of AB-PreS was assessed by inhibition ELISA test using sera allergic patients and from immunized rabbits. Results: IgE-binding experiments and basophil activation test revealed the hypoallergenic nature of AB-PreS. IgG antibodies induced by 5 injections with AB-PreS inhibited allergic patients’ IgE binding to Bet v 1 and Mal d 1 better than did IgG induced by up to 30 injections of six licensed birch pollen allergen extract-based vaccines. Additionally, immunization with AB-PreS induced HBV-specific antibodies potentially protecting the infection. Conclusion: The recombinant AB-PreS-based vaccine is hypoallergenic, safe and superior to currently registered allergen extract-based vaccines for the treatment of the birch pollen food allergy syndrome. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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