EXPRESSION OF I-A?kAND ALLELIC RESTRICTION ON A?/A?PAIRING IN TRANSGENIC MICE
| Autor: | Suresh Savarirayan, J. Martin, Chella S. David, B. Y. Wei |
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| Rok vydání: | 1990 |
| Předmět: | |
| Zdroj: | European Journal of Immunogenetics. 17:151-159 |
| ISSN: | 1365-2370 0960-7420 |
| DOI: | 10.1111/j.1744-313x.1990.tb00867.x |
| Popis: | Cell surface expression of class 11 major histocompatibility complex encoded (1a) molecules depends on association of the component alpha and beta chain into a stable heterodimer. Studies on cell lines transfected with MHC class II genes have revealed important limitations on the assembly of haplotype-mismatched A alpha:A beta complex. In order to study alpha:beta chain pairing restrictions in vivo a number of lines of transgenic mice carrying the A alpha k gene were generated. The transgene was studied in the context of H-2b, H-2s, H-2q, H-2u, H-2f and H-2v haplotypes. Initial FACS analysis of spleen and peripheral blood cells showed no A alpha kappa expression. Spleen cells stimulated with LPS and analysed by FACS showed A alpha k expression in three different H-2b strains as well as H-2u, but not in others. These data indicate that A alpha k can associate with A beta b and A alpha k, but not with A beta s, A beta q, A beta f, A beta v. |
| Databáze: | OpenAIRE |
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