Virus-induced silencing of a tobacco deoxyhypusine synthase gene

Autor: Wang Guoying, Wang Hongzhi, Wei JianHua, Lin Rui-Fen, Ma RongCai
Rok vydání: 2005
Předmět:
Zdroj: Chinese Science Bulletin. 50:2707-2713
ISSN: 1861-9541
1001-6538
DOI: 10.1007/bf02899640
Popis: A cDNA fragment corresponding to deoxyhypusine synthase geneNbDHS was isolated and cloned into potato virus X (PVX) vector for functional analysis inNicotiana benthamiana by using virus-induced gene silencing (VIGS). Plants agroinfected with recombinant virus vector PVX-NbDHS exhibited an increase in leaf biomass, delay in natural leaf senescence and flowering time, and decrease in leaf chlorophyll content. Semi-quantitative RT-PCR and Northern analysis showed that the transcript level ofDHS was significantly lower in PVX-NbDHS infected plants. At the same time, the expression for eIF-5A, the target proteins of DHS inN. benthamiana, was concomitantly suppressed by semi-quantitative RT-PCR and Western analysis. From the phenotypic feature of the infected plants and the reduced expression abundance ofDHS andeIF-5A, we concluded thatNbDHS plays important roles in plant growth, development and senescence. The possible application ofDHS gene in genetic modification of crops and horticultural plants was discussed.
Databáze: OpenAIRE
Externí odkaz: