Improved 4D NMR experiments for the assignment of backbone nuclei in13C/15N labelled proteins

Autor:	Sharon Campbell-Burk, Peter J. Domaille, Wayne Boucher, Ernest D. Laue
Rok vydání:	1992
Předmět:	Crystallography Chemistry Constant (mathematics) Biochemistry Combinatorial chemistry Spectroscopy
Zdroj:	Journal of Biomolecular NMR. 2:631-637
ISSN:	1573-5001 0925-2738
DOI:	10.1007/bf02192851
Popis:	We recently proposed a novel 4D NMR strategy for the assignment of backbone nuclei in13C/15N-labelled proteins (Boucher et al., 1992). Intra-residue (and many sequential) assignments are obtained from a HCANNH experiment, whereas sequential assignments are based on a complementary HCA(CO)NNH experiment. We present here new constant time 4D HCANNH, HCA(CO)NNH and HNCAHA experiments that are more sensitive. Some of the data were presented at the 33rd ENC held at Asilomar, California, U.S.A., in April 1992.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e80f9ab21a807868df5e9657b591d5de https://doi.org/10.1007/bf02192851 Zobrazit plný text záznamu Full text from SpringerLink