31P NMR Measurement of Intracellular pH in Bacillus FTU

Autor:	A. N. Belozersky, M. L. Verhovskaya, T. L. Knubovets
Rok vydání:	1990
Předmět:	Chromatography biology Chemistry Microorganism Intracellular pH Organic Chemistry Analytical chemistry chemistry.chemical_element Bacillus biology.organism_classification Biochemistry Oxygen Inorganic Chemistry chemistry.chemical_compound pH indicator Extracellular Intracellular Test tube
Zdroj:	Phosphorus, Sulfur, and Silicon and the Related Elements. 51:415-415
ISSN:	1563-5325 1042-6507
DOI:	10.1080/10426509008040942
Popis:	The ability of alkalotolerant bacterium Zacillus FTU to maintain intracellular pH was studied in the wide range of extracellular pH: 6.5–11, by means of 31P NMR. Bacillus FTU is an obligatory aerobic microorganism, possessing a high speed of oxygen consumption, that is why it was especially important to provide sufficient oxygen supply during the entire period of measurement. We have worked out a special glass filter insert for the NMR sample tube which allowed for uniform air supply in the cell suspension. The viability of the cells was checked by ATP level analysis Since in the case of Bacillus FTU ATP content if highly sensitive to the quantity of oxygen. The endogeneous P1 was used as a pH indicator. The level of Pi is extremely high in Bacillus FTU-around 70–30 mM, that's why it was possible to use relatively low concentration of cells in the suspension to obtain sufficient NMR sensitivity (approximately 5–7; of intracellular volume). Special procedure was employed to obtain a calibration cu...
Databáze:	OpenAIRE
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