Sensitive detection of thec-KITc.1430G>T mutation by mutant-specific polymerase chain reaction in feline mast cell tumours

Autor:	M Sato, M Takanosu, Y Kagawa
Rok vydání:	2012
Předmět:	General Veterinary Sequence analysis Point mutation Mutant Mastocytoma Biology medicine.disease Mast cell Molecular biology law.invention genomic DNA medicine.anatomical_structure law medicine Restriction fragment length polymorphism Polymerase chain reaction
Zdroj:	Veterinary and Comparative Oncology. 12:138-142
ISSN:	1476-5810
DOI:	10.1111/j.1476-5829.2012.00346.x
Popis:	Here, we describe the establishment of mutant-specific polymerase chain reaction (PCR) for detection of a c-KIT c.1430G>T mutation in feline mast cell tumours. Several mutations in feline c-KIT have been identified, with the c.1430G>T mutation accounting for a significant portion of feline mast cell tumour mutations. The c.1430G>T mutation in c-KIT exon 9 was detected in 15.7% (11 of 70) of samples by mutant-specific PCR but in only 7.1% (5 of 70) by PCR-restriction fragment length polymorphism (RFLP) in the genomic DNA isolated from 70 formalin-fixed paraffin-embedded sections or cells collected by fine needle aspiration. Mutant-specific PCR showed remarkably higher detection rate than did PCR-RFLP. DNA sequence analysis did not always yield identical results to those of mutant-specific PCR, suggesting heterogeneity of tumour cells. Mutant-specific PCR is a valid and efficient screening tool for detection of the c-KIT c.1430G>T point mutation in feline mast cell tumours compared with PCR-RFLP and sequencing analysis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e8001d45013c17a3ed73f0de993e9b0a https://doi.org/10.1111/j.1476-5829.2012.00346.x Zobrazit plný text záznamu Plný text