| Autor: |
Dan Wang, Katsunobu Yamamoto, Haihong Xu, Litong Jin, Jun Ye, Wen Zhang, Wei Zhu |
| Rok vydání: |
2005 |
| Předmět: |
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| Zdroj: |
Analytica Chimica Acta. 545:182-188 |
| ISSN: |
0003-2670 |
| DOI: |
10.1016/j.aca.2005.04.089 |
| Popis: |
The fabrication and application of a novel electrochemical detection (ED) system with the platinum/poly(methyl violet) (Pt/MV) chemically modified electrode (CME) for high performance liquid chromatography (HPLC) were described. The Pt particles deposited on the poly-MV film were characterized by atomic force microscope (AFM). It was found that the Pt/MV CME exhibited efficiently electrocatalytic effect on the current responses of cysteine (Cys), homocysteine (Hcy) and methionine (Met) with relatively high sensitivity, stability and long-life of activity. In HPLC-ED, these three amino acids had good and stable current responses at the CME and their linear ranges were over three orders of magnitude ( R ≥ 0.9996) with the detection limits being 7.5 × 10 −8 mol L −1 for Cys, 1.0 × 10 −7 mol L −1 for Hcy, 5.0 × 10 −7 mol L −1 for Met. The application of this method coupled with microdialysis sampling for the determination of Cys, Hcy and Met in plasma from patients with hypothyroidism was satisfactory. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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