Molecular Cloning, Tissue Distribution and Expression of Porcine y+L Amino Acid Transporter-1

Autor:	Xiangyan Zhou, Shigeng Zou, Zhiyi Huang, Lin Tao, Dingyuan Feng, Aimin Zhi, Jianjun Zuo, Xiao-lan Wang
Rok vydání:	2009
Předmět:	chemistry.chemical_classification Messenger RNA Biology Molecular biology Amino acid Biochemistry chemistry Rapid amplification of cDNA ends Complementary DNA Gene expression Animal Science and Zoology Amino acid transporter Peptide sequence Cellular localization Food Science
Zdroj:	Asian-Australasian Journal of Animal Sciences. 23:272-278
ISSN:	1976-5517 1011-2367
DOI:	10.5713/ajas.2010.90275
Popis:	In this study, we cloned, sequenced and characterized porcine y+L Amino Acid Transporter-1 (y+LAT1). By screening a translated EST database with the protein sequence of the human y + LAT1 and by using rapid amplification of cDNA ends (RACE), the full-length cDNA encoding porcine y + LAT1 was isolated from porcine intestine RNA. It was 2,111 bp long, encoding a 511 amino acid trans-membrane glycoprotein composed of 12 transmembrane domains. The predicted amino acid sequence was found to be 91%, 90%, 87% and 87% identical to those of cattle, human, mouse and rat y + LAT1 respectively. Real-time RT-PCR results indicated that the small intestine had the highest y + LAT1 mRNA abundance and the lung had the lowest y + LAT1 mRNA abundance. Baby hamster kidney (BHK) cells transfected with green fluorescent protein (GFP) tagged porcine y + LAT1 cDNA indicated that the cellular localization of the gene product in BHK was on the plasma membrane.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e7f7ead2be65e6b28bb61678d2ca87c9 https://doi.org/10.5713/ajas.2010.90275 Zobrazit plný text záznamu