Ascorbate in the apoplast of elongating plant cells

Autor:	Aleksandra Romanova, E. I. Sharova
Rok vydání:	2018
Předmět:	biology Plant cell Ascorbic acid Redox General Biochemistry Genetics and Molecular Biology Apoplast Cell wall chemistry.chemical_compound chemistry biology.protein Biophysics Secretion General Agricultural and Biological Sciences Hydrogen peroxide Peroxidase
Zdroj:	Biological Communications. 63:77-86
ISSN:	2587-5779 2542-2154
Popis:	It was shown that basipetal retardation of cell elongation in the growth zone of etiolated maize mesocotyls correlates with a steep decrease in the apoplastic ascorbic acid (ASC) concentration (50 μM → 10 μM) and ascorbate redox state (17 % → 5 %). Exogenous ASC (0.3 mM) not only inhibits peroxidase-dependent oxidation of phenols in vitro. It also exerts a highly specific effect on the secretion of peroxidases by stimulating the release of some isoforms while inhibiting the release of others to the cell walls. This effect points to the hypothetic signaling function of apoplastic ascorbate. Previously, we described a basipetal decrease in hydrogen peroxide concentration in the apoplast (from 5.1 to 2.0 μM) and a two-times increase in cell wall peroxidase potential activity (Sharova et al., 2012). Summarizing found gradients, we can assume that the conditions in the apoplast of the upper mesocotyl segment are favorable for the occurrence of the Fenton reaction (high ASC and hydrogen peroxide concentrations) and unfavorable for the oxidation of phenols (high ASC concentration and low potential peroxidase activity), which contributes to cell wall extensibility and rapid cell elongation.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e7e88cc02216a10421d1e6f170cdf8e5 https://doi.org/10.21638/spbu03.2018.109 Zobrazit plný text záznamu