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Summary Among the classical techniques for protein purification, affinity chromatography allows a several-fold purification from dilute preparations in a single step. At this time, the user can choose among a large variety of sorbents with different affinity ligands for almost any protein purification problem. However, we must clearly distinguish between applications in laboratory experiments and on an industrial scale. Whereas no restrictions apply in laboratory applications as to the type of ligand, its stability, or its origin, industrial applications require validated and certified affinity sorbents which are robust under process conditions, including cleaning-in-place. Future developments will concentrate on highly selective affinity ligands, tailored for a particular application, which are suitable for the elimination of by-products. For industrial applications, especially in the pharmaceutical sector, validation will be a problem, but one that can be solved during product development. Thus, the future seems bright for affinity sorbents based on synthetic ligands, whatever their chemical structure will be, as long as they are stable under industrial operating conditions. |
