Metabolic capacities of in vitro alternatives for chemical testing in skin: Insights from the Colipa skin metabolism project

Autor:	Camilla Pease, Christine Götz, Ulrike Hübenthal, Ellen Fritsche, Robert Edwards, Carsten Goebel, Pierre Aeby, Paul L. Carmichael, Roland Pfeiffer, Karsten Ruwiedel
Rok vydání:	2009
Předmět:	chemistry.chemical_classification integumentary system Human skin General Medicine Metabolism Pharmacology Toxicology In vitro Cytosol Enzyme medicine.anatomical_structure chemistry Biochemistry Cell culture Microsome medicine Keratinocyte
Zdroj:	Toxicology Letters. 189:S70
ISSN:	0378-4274
DOI:	10.1016/j.toxlet.2009.06.209
Popis:	Therefore, the aim of this study is to characterize enzymatic activity of human skin compared to the following skin cell-derived in vitro models: keratinocyte-based cell lines, primary keratinocytes and a three-dimensional epidermal model. Skin was processed to yield cytosolic and microsomal extracts, while the epidermal model was examined in intact form as well as in cytosolic and microsomal preparations.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e7b6df1bffed699210b7f9f974f70490 https://doi.org/10.1016/j.toxlet.2009.06.209 Zobrazit plný text záznamu Full Text from ScienceDirect