Investigation of Glucose 6-Phosphate Production and Simultaneous Carboxy-Terminated PAMAM-Bound Adenine Nucleotides Regeneration by Conjugated Enzymes in a Batch Reactor

Autor:	Wael Abdelmoez, Haruo Ishikawa, Kosaku Ishimi, Iulian Bobe
Rok vydání:	2004
Předmět:	chemistry.chemical_classification Acetate kinase biology Glucokinase Stereochemistry General Chemical Engineering Batch reactor General Chemistry Cofactor chemistry.chemical_compound Enzyme Glucose 6-phosphate chemistry Adenine nucleotide Dendrimer biology.protein
Zdroj:	JOURNAL OF CHEMICAL ENGINEERING OF JAPAN. 37:1303-1309
ISSN:	1881-1299 0021-9592
DOI:	10.1252/jcej.37.1303
Popis:	The production of glucose 6-phosphate (G6P) and simultaneous carboxy-terminated PAMAM-bound adenine nucleotides regeneration by the conjugated enzymes, Bacillus stearothermophilus glucokinase (GK) and acetate kinase (AK), in a small batch reactor were investigated both theoretically and experimentally. As the cofactor, a new family of polymer-bound adenine nucleotides (ATP and ADP) was used which was prepared in our previous work (Abdelmoez et al., 2002) by binding native ATP or ADP to a carboxy-terminated polyamidoamine (PAMAM) dendrimer (polymer-bound ATP or ADP). The experimental time courses of the glucose conversion were in a good agreement with the theoretical predictions calculated using the rate equations of G6P synthesis catalyzed by B. stearothermophilus GK (Abdelmoez et al., 2004) and the simplified rate equation of polymer-bound ATP regeneration catalyzed by B. stearothermophilus (AK) which is proposed in the present work.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e7b5869e2df2d664b7c1015223522f94 https://doi.org/10.1252/jcej.37.1303 Zobrazit plný text záznamu