Immunologic and Immunochemical Studies in the Hamster
| Autor: | Ruth A. Fugmann, M. Michael Sigel |
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| Rok vydání: | 1968 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 100:1101-1111 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.100.5.1101 |
| Popis: | Summary The capability of the hamster to produce 19 S antibody was demonstrated. The presence, amount and persistence of this immunoglobulin in hamster serum appeared to be markedly influenced by the antigen used for immunization. Antibody of the 19 S class was detected in animals immunized with influenza virus and with sheep erythrocytes. In the former case it was present in significant amounts only in the early phase of immunization, whereas in the latter case hemolytic activity associated with this antibody predominated in all antisera examined. No 19 S antibody was found in anti-bovine γ-globulin sera. The presence of two populations of 7 S immunoglobulins of differing electrical charge was demonstrated in immune sera following immunization with sheep erythrocytes, with influenza virus, and with bovine γ-globulin. These antibodies could not be distinguished by sucrose gradient centrifugation, treatment with 2-mercaptoethanol, nor by their reactivity in passive cutaneous anaphylaxis and complement-fixation tests. There was an indication that the faster migrating of the two IgG populations differed from the other by virtue of greater agglutinating capacity. |
| Databáze: | OpenAIRE |
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