Differential Effects of In Vitro Treatment with Cinobufotalin on Three Types of Ovarian Cancer Cells
| Autor: | Anthony B. Mcdowell, Chander Peddaboina, Mohammad N. Uddin, M. Karen Newell-Rogers, David C. Zawieja, A.H.M. Zuberi Ashraf, Timothy C. Mccormick, Syeda H. Afroze, Thomas J. Kuehl |
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| Rok vydání: | 2018 |
| Předmět: |
Cancer Research
Cell type biology medicine.diagnostic_test Cell growth General Medicine Cell sorting Immunofluorescence Molecular biology Proliferating cell nuclear antigen chemistry.chemical_compound Oncology chemistry Cell culture biology.protein medicine Viability assay Fluorescein isothiocyanate |
| Zdroj: | Anticancer Research. 38:5717-5724 |
| ISSN: | 1791-7530 0250-7005 |
| DOI: | 10.21873/anticanres.12909 |
| Popis: | Background/aim Cinobufotalin (CINO), a cardiotonic steroid, has been used as an anticancer agent. This study assessed the cell-specific effect of CINO on SK-OV-3, CRL-1978 and CRL-11731 ovarian cancer cells which differ in terms of their respective karyotypes. Materials and methods Cell cultures were treated with CINO (0.1, 1, 5 and 10 μM) for 24, 48, and 72 h. Cell proliferation, migration, and invasion were measured using CellTiter, Cytoselect, and FluoroBlock assays, respectively. Expression of proliferating cell nuclear antigen (PCNA) was evaluated by western blot analysis. Cell viability was determined by fluorescence-activated cell sorting. Immunofluorescence was performed using Annexin-V staining and fluorescein isothiocyanate (FITC). Mitochondrial membrane potential (MMP) was measured using MitoTracker™ Red. Results CINO at 0.5 μM inhibited SK-OV-3, CRL-1978, and CRL-11731 proliferation, migration, and invasion. Each cell type differed in response to CINO doses for PCNA, Annexin-V expression and MMP. Conclusion The antineoplastic property of CINO is consistent, but its mode of action varies among cell lines. |
| Databáze: | OpenAIRE |
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