Characterization of Carotenoid Triplet States in the Light-Harvesting Complex B800–850from the Purple Bacterium Rubrivivax gelationsus
| Autor: | Arnold J. Hoff, Gabrielle M. Owen, Vladimira Jirsakova, Françoise Reiss-Husson, Bas van Dijk |
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| Rok vydání: | 1996 |
| Předmět: |
chemistry.chemical_classification
Hplc analysis biology Chemistry General Medicine Photochemistry biology.organism_classification Polyene Biochemistry Light-harvesting complex Protein environment chemistry.chemical_compound Physical and Theoretical Chemistry Spectroscopy Carotenoid Neurosporene Bacteria |
| Zdroj: | Photochemistry and Photobiology. 64:363-368 |
| ISSN: | 1751-1097 0031-8655 |
| DOI: | 10.1111/j.1751-1097.1996.tb02472.x |
| Popis: | The carotenoid triplet states in the light-harvesting complex B800–850from purple bacterium Rubrivivax gelatinosus were characterized by absorption-detected magnetic resonance in zero magnetic field (ADMR) spectroscopy. Detailed HPLC analysis of carotenoids from B800–850demonstrated the presence of several carotenoids bound to the complex: the major ones are hydroxyspheroidene and spheroidene (together 80%), followed by neurosporene and hydroxyneurosporene (7%), spheroidenone and hydroxyspheroidenone (7.5%) and two other minor carotenoids that could be 3,4-dihydrospheroidenone and 3,4-dihydrohydroxyspheroidenone (5.5%). Three triplet states originating from carotenoids present in the B800–850were observed. The identical T-S spectra recorded at selectively chosen 2|E| transitions of carotenoids indicated that all these triplet states can be attributed to three different populations of one carotenoid family, probably to spheroidene and to hydroxyspheroidene, with different out-of-plane distortions of their polyene chain due to a different protein environment. Triplet states of the neurosporene and the spheroidenone families are probably not observed because of the low signal amplitude. |
| Databáze: | OpenAIRE |
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