| Autor: |
M. D. Russett, A. J. Adler, E. A. Dratz, D. M. Snodderly, Garry J. Handelman |
| Rok vydání: |
1992 |
| Předmět: |
|
| DOI: |
10.1016/0076-6879(92)13123-f |
| Popis: |
Publisher Summary This chapter discusses the dissection method of measurement of carotenoids in human and monkey retinas. Proficiency with the dissection method requires practice and familiarity with the structure of the eyeball. The method takes approximately 30 min/specimen, and eight specimens per day can reasonably be dissected. After removal from the eyecup and separation of the macula from the retina, the specimens can be safely frozen at -80 ° C in buffer for subsequent high-performance liquid chromatographic (HPLC) analysis. If the sample is stored frozen, the vial should be rinsed well with 1.0 ml of ethanol-buffer (50:50, v/v) to pick up fragments of tissue that might be left in the vial. This additional rinse liquid should be homogenized and pooled with the rest of the homogenate. The total volumes in this case may need to be adjusted to maintain the solvent proportions of one part ethanol to one part buffer to four parts hexane. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
