Surface plasmon resonance biosensor for the detection of tau-amyloid β complex
| Autor: | Erika Hemmerová, Zdenka Kristofikova, Giusy Finocchiaro, Tomáš Špringer, Jiří Homola, Martin Vyhnalek |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Detection limit
Amyloid β biology Chemistry Tau protein Metals and Alloys Surface plasmon resonance biosensor 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics 01 natural sciences 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Colloidal gold Materials Chemistry Biophysics biology.protein Surface modification Electrical and Electronic Engineering Surface plasmon resonance 0210 nano-technology Instrumentation Biosensor |
| Zdroj: | Sensors and Actuators B: Chemical. 316:128146 |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/j.snb.2020.128146 |
| Popis: | The development of methods advancing the understanding of molecular processes related to Alzheimer’s disease (AD) and detection of biomarkers of AD presents an important research goal. We present a new biosensor-based method for the detection of a prospective biomarker of AD, the complex of protein tau and amyloid β (tau-Aβ). The detection method combines a new sandwich assay incorporating functionalized gold nanoparticles (AuNPs) with a surface plasmon resonance (SPR) biosensor. We optimize the assay with respect to the functionalization of the sensor surface and the choice of AuNPs and show that the sensor allows for the detection of the tau-Aβ complex in cerebrospinal fluid (CSF) with the limit of detection of 1 pM. We also present the results of a preliminary study in which we use the method to detect tau-Aβ complex in CSF samples. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect