A Rapid One-Step Immunoradiometric Assay for Factor VIII-Procoagulant Antigen Utilizing Monoclonal Antibodies

Autor:	van Mourik Ja, Stel Hv, Veerman Ec, Janssen Mc, Huisman Jg
Rok vydání:	1983
Předmět:	Immunoradiometric assay biology Antigen medicine.drug_class Chemistry medicine biology.protein Hematology Antibody Monoclonal antibody Molecular biology Incubation Lower limit
Zdroj:	Thrombosis and Haemostasis. 50:860-863
ISSN:	2567-689X 0340-6245
DOI:	10.1055/s-0038-1665329
Popis:	SummaryA two-site immunoradiometric assay for factor VIII-procoagulant antigen (VIIICAg) that relies completely on monoclonal antibodies has been developed. By selecting an appropriate combination of these antibodies, it was possible to develop an assay in which the radiolabelled monoclonal antibody did not inhibit the binding of antigen to the solid-phase monoclonal antibodies. Thus, the entire test could be carried out as a one-step procedure. With this one-step assay, an amount of 0.0025 U VIIICAg/ml plasma could be detected after 4 hr of incubation, whereas 18 hr of incubation resulted in a lower limit of sensitivity of 0.0005 U VIIICAg/ml. The use of a one-step assay provides a significant advantage over the conventional two-step assay by simplifying, shortening and rendering the performance of the assay more convenient.
Databáze:	OpenAIRE
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