Development and improved selected markers to biosurfactant and bioemulsifier production by Rhizopus strains isolated from Caatinga soil
| Autor: | Milagre Americo Pele, Daylin Rubio-Ribeaux, D. Montero-Rodríguez, Marcos Antônio Cavalcanti Luna, André L. C. M. A. Santiago, Galba Maria de Campos-Takaki, Michele F. Santiago, Thayse Alves de Lima e Silva, Rosileide F. S. Andrade, Adriana Ferreira Souza |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Rhizopus microsporus Parafilm biology Chemistry 030106 microbiology Rhizopus species Sheep blood agar biology.organism_classification medicine.disease Applied Microbiology and Biotechnology Hemolysis 03 medical and health sciences 030104 developmental biology Clear zone Rhizopus Genetics medicine Rhizopus arrhizus Food science Agronomy and Crop Science Molecular Biology Biotechnology |
| Zdroj: | African Journal of Biotechnology. 17:150-157 |
| ISSN: | 1684-5315 |
| Popis: | This study screened four Rhizopus species as biosurfactant producers using different markers. First of all, Rhizopus spp. UCP 1607 was identified as Rhizopus arrhizus by morphological and molecular methods. The production of biosurfactant/bioemulsifier was investigated by submerged fermentation using soybean post-frying oil (5% v/v) and sodium glutamate (1% w/v) medium. The primary markers’ hemolysis and parafilm M tests showed that R. arrhizus UCP1607 strain exhibited higher hemolytic activity (49 mm of clear zone) on sheep blood agar and a larger drop diameter (12 mm) on parafilm hydrophobic surface. The experimental results showed the most promising biosurfactant production by R. arrhizus UCP 1607 strain led to a reduction of surface tension (31.8 mN/m) and the diameter of the oil-spreading covered an area of 66.4 cm2. The strains Rhizopus microsporus var. chinensis UCP1296, R. microsporus var. microsporus UCP1304, and R. arrhizus UCP1607 were capable of forming stable emulsions corresponding to 91.7, 94.8, and 82.6%, respectively in crude oil. Key words: Tensio-active agent, bioemulsifier, screening of Rhizopus strains, submerged fermentation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|