Experimental Investigation of Poly-β-Hydroxybutyrate Production byAzohydromonas lata: Kinetics and Artificial Neural Network Modeling
| Autor: | F. Karbasi, Habibollah Younesi, H. Qaderi, M. Ardjmand, A.A. Safe kordi, S. Yaghmaei |
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| Rok vydání: | 2014 |
| Předmět: |
0106 biological sciences
General Chemical Engineering Kinetics chemistry.chemical_element General Chemistry 010501 environmental sciences 01 natural sciences Nitrogen Polyhydroxyalkanoates chemistry.chemical_compound chemistry 010608 biotechnology Proton NMR Ammonium chloride Fourier transform infrared spectroscopy Spectroscopy Chemical composition 0105 earth and related environmental sciences Nuclear chemistry |
| Zdroj: | Chemical Engineering Communications. 203:224-235 |
| ISSN: | 1563-5201 0098-6445 |
| DOI: | 10.1080/00986445.2014.990631 |
| Popis: | Batch culture of Azohydromonas lata was investigated for the production of intracellular poly-β-hydroxybutyrate (PHB). In order to determine the C:N value of the culture media for maximizing the microbial productivity of PHB, different concentrations of glucose and ammonium chloride were used as carbon and nitrogen sources, respectively. The optimal temperature and shaking rate was obtained at 30°C and 180 rpm, respectively. The maximum intracellular PHB concentration obtained was 5.09 g/l, which was 20% (w/w) of the cell dry weight (CDW) after 72 h. Also, the synthesis of PHB was growth associated with the C:N ratio of 153.71. The maximum calculated Yp/s was 0.212 (gr/gr) and the specific production rate value after 12 h was 0.264 g/l/h, with 40 and 50 g/l of glucose concentrations, respectively, with 0.5 g/l ammonium chloride kept constant. The chemical composition of the resulting PHB was analyzed by Fourier transform infrared spectroscopy and proton nuclear magnetic resonance spectroscopy. The Leudeki... |
| Databáze: | OpenAIRE |
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