Bothrojaracin, a Bothrops jararaca Snake Venom-Derived (Pro)Thrombin Inhibitor, as an Anti-Thrombotic Molecule

Autor:	Russolina B. Zingali, Robson Q. Monteiro, Mariane Assafim, Flávia S. Frattani, Marcos S. Ferreira
Rok vydání:	2005
Předmět:	Bothrops jararaca biology business.industry Hematology Pharmacology medicine.disease biology.organism_classification Venous thrombosis Thrombin Snake venom In vivo Physiology (medical) Immunology medicine Bothrojaracin Thrombus business medicine.drug
Zdroj:	Pathophysiology of Haemostasis and Thrombosis. 34:160-163
ISSN:	1424-8840 1424-8832
DOI:	10.1159/000092416
Popis:	Bothrojaracin (BJC) is a selective and potent thrombin inhibitor (KD = 0.6 nM) which also binds to prothrombin on proexosite I (KD = 175 nM). Incubation of BJC with human or rat plasma produced a band that co-migrates with purified prothrombin-BJC complex. We further analyzed the in vivo anti-thrombotic effect of BJC on a venous thrombosis model in rats that combines stasis and hypercoagulability. The administration of 1 mg/kg (i.v.) doses of BJC decreased thrombus weight by ∼95%. Evaluation of the in vivo effect of BJC in mice using a pulmonary thromboembolism model induced by thrombin showed that BJC protects 100% of mice from death. Altogether, our data show that BJC is a potent anti-thrombotic agent that could further help the development of new prothrombin-directed drugs.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e771773688c094abe6015e0fca9d1e7a https://doi.org/10.1159/000092416 Zobrazit plný text záznamu