Inhibition of biofilm formation by chemical uncoupler, 3, 3′, 4′, 5-tetrachlorosalicylanilide (TCS): From the perspective of quorum sensing and biofilm related genes
| Autor: | Xiaochi Feng, Haichao Luo, Qinglian Wu, Heshan Zheng, Nanqi Ren, Wanqian Guo, Juanshan Du |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Environmental Engineering Chemistry fungi Biofilm inhibition Biomedical Engineering Biofilm Bioengineering biochemical phenomena metabolism and nutrition 010501 environmental sciences Inhibitory postsynaptic potential 01 natural sciences Cell biology Extracellular matrix 03 medical and health sciences Quorum sensing 030104 developmental biology Real-time polymerase chain reaction Downregulation and upregulation Gene 0105 earth and related environmental sciences Biotechnology |
| Zdroj: | Biochemical Engineering Journal. 137:95-99 |
| ISSN: | 1369-703X |
| DOI: | 10.1016/j.bej.2018.05.010 |
| Popis: | Biological anti-biofouling strategy based on chemical uncoupler is considered as a potential alternative in membrane filtration systems. However, the inhibitory mechanism of uncoupler on biofilm development remains to be uncovered. To bridge the knowledge gap, this study investigated the inhibitory effectiveness of TCS on a gram-positive bacterial biofilm formation and analyzed the genes expression associated with quorum sensing (QS) system and biofilm formation via real-time quantitative polymerase chain reaction (RT-qPCR). TCS was observed to inhibit the biofilm formation of B. subtilis as low as 100 μg/L. The expression of genes associated with ComX QS system were not changed. However, the sinR and abrB genes which were modulated by CSF QS system, were upregulated by 100 μg/L TCS. The SinR protein negative regulated genes (epsE, tasA and yqxM) involved in extracellular matrix production were repressed, corroborating the reduction of extracellular matrix was the main cause of biofilm inhibition by TCS. |
| Databáze: | OpenAIRE |
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