The inhibitory effect of somatostatin on gastric motility in Suncus murinus
| Autor: | Takafumi Sakai, Ichiro Sakata, Naho Yokota, Keiji Nakayama, Shota Takemi, Hiroki Okada, Haruka Sekiya |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
endocrine system medicine.medical_specialty Physiology medicine.drug_class Gastric motility Motility 030209 endocrinology & metabolism Motilin 03 medical and health sciences 0302 clinical medicine In vivo Internal medicine parasitic diseases medicine biology Chemistry digestive oral and skin physiology General Medicine Suncus Receptor antagonist biology.organism_classification 030104 developmental biology Endocrinology Somatostatin hormones hormone substitutes and hormone antagonists Abnormal gastrointestinal motility |
| Zdroj: | Journal of Smooth Muscle Research. 56:69-81 |
| ISSN: | 1884-8796 0916-8737 |
| DOI: | 10.1540/jsmr.56.69 |
| Popis: | Gastric contractions show two specific patterns in many species, migrating motor contractions (MMC) and postprandial contractions (PPCs), that occur in the fasted and fed states, respectively. In this study, we examined the role of somatostatin (SST) in gastric motility both in vivo and in vitro using the Asian house shrew (Suncus murinus). We performed in vivo recordings of gastric motility and in vitro organ bath experiments using S. murinus, which was recently established as a small laboratory animal for use in tests of gastrointestinal motility. SST (1.65 µg kg-1 min-1) was intravenously administered during phase II of MMC and PPCs. Next, the effect of SST on motilin-induced gastric contractions at phase I of MMC was measured. Cyclosomatostatin (CSST), an SST receptor antagonist, was administered at the peak of phase III of MMC. In addition, the effect of SST (10-11-10-9 M) on motilin-induced gastric contractions was evaluated using an organ bath experiment in vitro. In conscious, free-moving S. murinus, the administration of SST decreased the occurrence of the spontaneous phase II of MMC and PPCs. Pretreatment with SST and octreotide suppressed the induction of motilin-induced gastric contractions both in vivo and in vitro. Administration of CSST before the peak of spontaneous phase III contractions had no effect on gastric contractions. Endogenous SST is not involved in the regulation of gastric MMC and PPCs, but exogenous SST suppresses spontaneous gastric contractions. Thus, SST would be good for treating abnormal gastrointestinal motility disorders. |
| Databáze: | OpenAIRE |
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