Mechanisms of Coupling between Angiotensin Converting Enzyme 2 and Nicotinic Acetylcholine Receptors
| Autor: | Nadine Kabbani, Kyle D. Brumfield, Patricia Sinclair, Arvind Ramanathan, Menu Leddy, Rita R. Colwell, James L Olds |
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| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
Rabies virus medicine.disease_cause Cell biology Nicotine Nicotinic agonist nervous system chemistry Cell culture Angiotensin-converting enzyme 2 medicine Neurotoxin Glycoprotein hormones hormone substitutes and hormone antagonists medicine.drug Acetylcholine receptor |
| DOI: | 10.1101/2021.08.12.456154 |
| Popis: | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), an RNA virus encapsulated by a spike (S) glycoprotein envelope, binds with high affinity to angiotensin converting enzyme 2 (ACE2) during cell entry of a susceptible host. Recent studies suggest nicotinic acetylcholine receptors (nAChRs) play a role in functional ACE2 regulation and nicotine may contribute to the progression of coronavirus disease 2019 (COVID-19). Here, we present evidence for coupling between ACE2 and nAChR through bioinformatic analysis and cell culture experiments. Following molecular and structural protein comparison of over 250 ACE2 vertebrate orthologues, a region of human ACE2 at positions C542-L554 was identified to have sequence similarity to nAChR-binding neurotoxin and rabies virus glycoproteins (RBVG). Furthermore, experiments conducted in PC12 cells indicate a potential for physical interaction between ACE2 and α7 nAChR proteins. Our findings support a model of nAChR involvement in in COVID-19. |
| Databáze: | OpenAIRE |
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