A Simple Flow Amperometric Electrochemical Biosensor Based on Chitosan Scaffolds and Gold Nanowires Modified on a Glassy Carbon Electrode for Detection of Glutamate in Food Products
| Autor: | Jiraporn Kitikul, Anchana Preechaworapun, Tanin Tangkuaram, Pusit Pookmanee, Sakchai Satienperakul |
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| Rok vydání: | 2016 |
| Předmět: |
Flow injection analysis
Detection limit Chemistry Scanning electron microscope 010401 analytical chemistry Analytical chemistry 02 engineering and technology Carbon nanotube 021001 nanoscience & nanotechnology 01 natural sciences Amperometry 0104 chemical sciences Analytical Chemistry law.invention Chitosan chemistry.chemical_compound Membrane law Electrochemistry 0210 nano-technology Biosensor Nuclear chemistry |
| Zdroj: | Electroanalysis. 29:264-271 |
| ISSN: | 1040-0397 |
| DOI: | 10.1002/elan.201600263 |
| Popis: | A glutamate biosensor based on cross-linked glutamate oxidase with chitosan (CHIT) on a carbon nanotube (CNT)-modified gold nanowire (AuNW) was constructed and applied in a flow injection analysis (FIA) system. The AuNW was produced using an alumina template membrane and characterized using scanning electron microscopy (SEM) with a 120-nm diameter. The CHIT cross-linked CNT slurry was mixed with AuNW and cast on the surface of a glassy carbon electrode (GCE), followed by immobilization with the glutamate oxidase. Optimization of the experimental parameters, including the applied potential, enzyme loading, flow rate, type of buffer and pH, resulted in values of 0.7 V, 30 μL, 1.0 mL min−1, phosphate buffer, and pH 7.4, respectively. The analytical working curve was linear in the ranges from 10 to 100 (R2=0.9981) and 100 to 20,000 μM glutamate (R2=0.9821) with a low detection limit of 1.2 μM (S/N=3). A glutamate biosensor with an FIA system was used to determine glutamate in samples of Thai commercial food products. |
| Databáze: | OpenAIRE |
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