Studies in Tuberculosis. XI. Neutralization of Filterable Tubercle Toxin (T. F.) with Sheep Antiserum (Antitoxin)
| Autor: | Frederick Eberson |
|---|---|
| Rok vydání: | 1927 |
| Předmět: | |
| Zdroj: | Experimental Biology and Medicine. 24:802-805 |
| ISSN: | 1535-3699 1535-3702 |
| DOI: | 10.3181/00379727-24-3578 |
| Popis: | In continuation of the experiments reported previously,1, 2, 3 Berkfeld filtrates from broth cultures of tubercle bacilli were used in the systematic immunization of a sheep. These toxin filtrates, hereafter designated as T. F., were obtained by growing a virulent strain of human tubercle bacilli in glycerinated “hormone broth” for 18 to 21 days and filtering the culture through a Berkfeld “N” candle. The sheep was injected subcutaneously at weekly intervals with increasing doses of freshly prepared filtrate. After four months, neutralizing properties of the serum were studied by the intracutaneous and intraperitoneal method in tuberculous and normal guinea pigs, and by skin tests in tuberculous and non-tuberculous human subjects.Having found previously that an intracutaneous injection of unheated T. F. in the experimental tuberculous animal caused a definite skin reaction, the effect of an incubated mixture of immune sheep serum and T. F. was now studied.Intracutaneous Method. Series of four guinea pigs ... |
| Databáze: | OpenAIRE |
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