Characterization of glucocorticoid receptor in B lymphocytes (111.3)
| Autor: | Amanda Gruver-Yates, John Cidlowski |
|---|---|
| Rok vydání: | 2012 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 188:111.3-111.3 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.188.supp.111.3 |
| Popis: | Glucocorticoids play an important role in the resolution of inflammation. They are routinely used to treat allergies, asthma, sepsis, and autoimmune diseases. In addition to immune suppression, glucocorticoids can negatively impact the development and function of the immune system by inducing apoptosis, an effect well-studied in mature and developing T cells. Little is known however about glucocorticoid function specifically in B lymphocytes. We have shown that glucocorticoid receptor (GR) is present in enriched B cell populations isolated from mouse spleen. GR protein was present in all B cell (B220+) developmental subsets (Mature IgM+IgD+, Immature IgM+IgD-, and Pro/Pre IgM-IgD-) isolated from spleen. Intracellular staining for GR analyzed with flow cytometry also confirmed the presence of GR in all B cell subsets from cells isolated both from mouse spleen and bone marrow. Mean fluorescence intensity data indicated that Pro/Pre B lymphocytes had more intense staining of GR than either mature or immature populations. Ex vivo cell culture of murine splenocytes indicated that dexamethasone, a synthetic and potent glucocorticoid, reduced cell viability in culture in all B cell developmental subsets. In vivo administration of dexamethasone reduced total cell numbers of all B cell subsets present in the mouse spleen within 24 hours. These data suggest that B cells glucocorticoid signaling through GR on B cells have an important role in B cell function. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|