Popis: |
Potato plants infected by phytoplasmas exhibit an array of symptoms that suggest interference within the normal balance of plant metabolism. Suppression subtractive hybridization (SSH), macroarray screening and real-time PCR were performed to identify differentially expressed genes in phytoplasma-infected in vitro potato plants. Gene expression analysis revealed the up-regulation of genes involved in several metabolic pathways, including ERF , WRKY , HSP90 , SAP6 , AN1-like ZFP , and an unknown protein; the down-regulation of genes encoding monodehydroascorbate reductase and a putative membrane protein was also observed. The results presented here provide the groundwork for addressing new questions to better understand the phytoplasma–plant interaction. |