The Effect of Glutathione Depletion in the Glutamine Synthetase Gene Amplification System

Autor:	Daniel R. Omstead, Jane A. Reese, Shue-Yuan Wang, Robert Zivin
Rok vydání:	1993
Předmět:	Glutamine synthetase gene chemistry.chemical_compound Chemistry Dna encoding Chinese hamster ovary cell Glutamine synthetase Methionine Sulfoximine Transfection Glutathione Gene Molecular biology
Zdroj:	Bioproducts and Bioprocesses 2 ISBN: 9783642493621
DOI:	10.1007/978-3-642-49360-7_5
Popis:	The glutamine synthetase (GS) system is a novel gene amplification system in which vectors containing GS coding sequences are used as dominant markers amplifiable by selection with methionine sulfoximine (MSX). DNA encoding the desired protein is combined and co-amplified with the GS gene. Transfected cells are cultured in glutamine-free medium with 500 uM MSX to potentiate the amplification.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e70c6e91c7233ba79bb39009e6f2c455 https://doi.org/10.1007/978-3-642-49360-7_5 Zobrazit plný text záznamu