Impact of nano-TiO2 on horizontal transfer of resistance genes mediated by filamentous phage transduction
| Autor: | Chang Liu, Peng Lv, Xiao-Lin Ma, Yujie Feng, Xue Han, Fei Long, Xiang Xiao, Luguang Wang, Ming-Feng Yang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Membrane permeability biology Chemistry Materials Science (miscellaneous) Cell Periplasmic space 010501 environmental sciences Nano tio2 biology.organism_classification 01 natural sciences Transmembrane protein Cell biology Bacteriophage 03 medical and health sciences medicine.anatomical_structure Horizontal gene transfer medicine Gene 030304 developmental biology 0105 earth and related environmental sciences General Environmental Science |
| Zdroj: | Environmental Science: Nano. 7:1214-1224 |
| ISSN: | 2051-8161 2051-8153 |
| Popis: | The spread of antibiotic resistance genes (ARGs) in the environment has aroused growing concern for human health and ecological safety. Recent studies have confirmed that engineered nanomaterials can increase the environmental risk of ARG dissemination mediated by conjugation and transformation. However, whether nanomaterials can mediate the transductive transfer of ARGs by phage infection has not been reported. In this work, the effect of nano-TiO2 on the horizontal transfer of ARGs to E. coli TG1 through transduction by constructed phage gM13 was investigated. Our results showed that nano-TiO2 concentration, particle size, mating time and the ratio of gM13/TG1 could influence the transductive transfer of ARGs. Further studies revealed that nano-TiO2 could promote bacteriophage attachment on cell surfaces, which contributed to gM13 infection. Meanwhile, increasing membrane permeability induced by nano-TiO2 could alleviate transmembrane resistance, which facilitated the infectious entry of phage gM13 into periplasmic space. The expressions of pilus-related genes were improved when E. coli TG1 was exposed to nano-TiO2. Thus, this work is beneficial for gaining a better understanding about the multiple environmental effects of increasingly released nanomaterials and is useful for the control of ARG dissemination in the environment. |
| Databáze: | OpenAIRE |
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