Modified Steiner for the Demonstration of Spirochetes

Autor:	Arleen Fathi, Winsome Garvey, Francine Bigelow
Rok vydání:	1985
Předmět:	Medical Laboratory Technology Silver nitrate chemistry.chemical_compound Mastic Gum Histology Tissue sections chemistry Uranyl nitrate Biochemistry Hydroquinone Modified technique Anatomy Nuclear chemistry
Zdroj:	Journal of Histotechnology. 8:15-17
ISSN:	2046-0236 0147-8885
DOI:	10.1179/his.1985.8.1.15
Popis:	Four histological techniques for the demonstration of spirochetes were evaluated. The Steiner technique(1944) was the most satisfactory but suffered a few drawbacks, such as precipitate and darkening of the tissue section which obscured the spirochetes. The method was therefore modified to improve on these points. The modified technique employs sensitization in uranyl nitrate, impregnation in 1% silver nitrate for an hour, brief exposure to 2.5% gum mastic followed by reduction in a solution composed of hydroquinone, gum mastic, absolute alcohol and a trace of silver nitrate. This method gives consistent results to reveal the presence of spirochetes and will demonstrate most bacteria as well.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e6dde648b6b1a48be697a232dc9bd26f https://doi.org/10.1179/his.1985.8.1.15 Zobrazit plný text záznamu