Finding Wolbachia in Filarial larvae and Culicidae Mosquitoes in Upper Egypt Governorate
Autor: | Yasser Mokhtar, Ahmed K. Dyab, Abeer E Mahmoud, Lamia A. Galal |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Aedes biology Culex fungi 030231 tropical medicine Dirofilaria immitis biochemical phenomena metabolism and nutrition biology.organism_classification medicine.disease_cause Virology Dirofilaria repens 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Infectious Diseases Wuchereria bancrofti Vector (epidemiology) parasitic diseases medicine bacteria Helminths Parasitology Wolbachia reproductive and urinary physiology |
Zdroj: | The Korean Journal of Parasitology. 54:265-272 |
ISSN: | 1738-0006 0023-4001 |
Popis: | Wolbachia is an obligatory intracellular endosymbiotic bacterium, present in over 20% of all insects altering insect reproductive capabilities and in a wide range of filarial worms which is essential for worm survival and reproduction. In Egypt, no available data were found about Wolbachia searching for it in either mosquitoes or filarial worms. Thus, we aimed to identify the possible concurrent presence of Wolbachia within different mosquitoes and filarial parasites, in Assiut Governorate, Egypt using multiplex PCR. Initially, 6 pools were detected positive for Wolbachia by single PCR. The simultaneous detection of Wolbachia and filarial parasites (Wuchereria bancrofti, Dirofilaria immitis, and Dirofilaria repens) by multiplex PCR was spotted in 5 out of 6 pools, with an overall estimated rate of infection (ERI) of 0.24%. Unexpectedly, the highest ERI (0.53%) was for Anopheles pharoensis with related Wolbachia and W. bancrofti, followed by Aedes (0.42%) and Culex (0.26%). We also observed that Wolbachia altered Culex spp. as a primary vector for W. bancrofti to be replaced by Anopheles sp. Wolbachia within filaria-infected mosquitoes in our locality gives a hope to use bacteria as a new control trend simultaneously targeting the vector and filarial parasites. |
Databáze: | OpenAIRE |
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