A Real-Time Fluorescence Polymerase Chain Reaction Assay for the Identification of Yersinia pestis Using a Field-Deployable Thermocycler

Autor:	William B. Huff, Andrew J. Rohrer, Mariana A. McConathy, Kenton L. Lohman, James C McAvin, William J. Barnes
Rok vydání:	2003
Předmět:	biology Public Health Environmental and Occupational Health General Medicine Yersinia biology.organism_classification Virology Genome Enterobacteriaceae law.invention genomic DNA chemistry.chemical_compound Yersinia pestis chemistry law TaqMan Polymerase chain reaction DNA
Zdroj:	Military Medicine. 168:852-855
ISSN:	1930-613X 0026-4075
Popis:	Real-time fluorescence polymerase chain reaction is a microbial identification method that can provide rapid and accurate results using a field-deployable thermocycler, the RAPID ("ruggedized" advanced pathogen identification device). A Yersinia pestis-specific TaqMan assay required approximately 75 minutes and achieved a sensitivity of 100 fg of Y. pestis genomic DNA (20 genome equivalents). Specificity testing against a genomic DNA cross-reaction panel comprised of 22 bacterial species encountered in the respiratory tract resulted in no false positives. No cross-reaction occurred with human genomic DNA.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e6c112ab42dde01d6769ddbe9018456f https://doi.org/10.1093/milmed/168.10.852 Zobrazit plný text záznamu Plný text ve formátu PDF