1H NMR and antioxidant profiles of polar and non-polar extracts of persimmon (Diospyros kaki L.) – Metabolomics study based on cultivars and origins
| Autor: | Yong Seo Park, Young Mo Kim, Yang-Kyun Park, Faridah Abas, M. Maulidiani, Shela Gorinstein, Ahmed Mediani |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
chemistry.chemical_classification
Lutein Antioxidant ABTS DPPH medicine.medical_treatment 010401 analytical chemistry food and beverages Diospyros kaki 04 agricultural and veterinary sciences 040401 food science 01 natural sciences 0104 chemical sciences Analytical Chemistry Zeaxanthin chemistry.chemical_compound 0404 agricultural biotechnology chemistry Polyphenol medicine Food science Carotenoid |
| Zdroj: | Talanta. 184:277-286 |
| ISSN: | 0039-9140 |
| Popis: | Persimmon (Diospyros kaki L.) is one of the most important fruits that has been consumed for its medicinal properties due to the presence of some active metabolites, particularly polyphenols and carotenoids. Previously described methods, including HPLC, were limited in the determination of metabolites in different persimmon varieties. The present study shows the evaluation and the differences among persimmon polar and non-polar extracts by 1H NMR-based metabolomics approach. The hierarchical clustering analysis (HCA) based on score values of principal component analysis (PCA) model was used to analyze the important compounds in investigated fruits. The 1H NMR spectrum of persimmon chloroform (CDCl3) extracts showed different types of compounds as compared to polar methanol-water (CD3OD-D2O) ones. Persimmons growing in Israel were clustered different from those growing in Korea with the abundance of phenolic compounds (gallic, caffeic and protocathecuic acids), carotenoids (β-cryptoxanthin, lutein, and zeaxanthin), amino acids (alanine), maltose, uridine, and fatty acids (myristic and palmitoleic acids). Glucose, choline and formic acid were more prominent in persimmon growing in Korea. In CD3OD-D2O and CDCl3 persimmon extracts, 43 metabolites were identified. The metabolic differences were shown as well on the results of bioactivities and antioxidant capacities determined by ABTS, FRAP, CUPRAC and DPPH assays. The presented methods can be widely used for quantitation of multiple compounds in many plant and biological samples especially in vegetables and fruits. |
| Databáze: | OpenAIRE |
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