Distribution of foreign mitochondrial DNA during the first splitting of transmitochondrial mouse embryos

Autor: Sorokin Av, M. E. Kustova, M. G. Bass, V. A. Sokolova, F. M. Zakharova, Vadim B. Vasilyev
Rok vydání: 2008
Předmět:
Zdroj: Cell and Tissue Biology. 2:609-613
ISSN: 1990-5203
1990-519X
DOI: 10.1134/s1990519x08060072
Popis: The distribution of human mitochondrial DNA (mtDNA) among single murine blastomeres was analyzed during the splitting of embryos injected with a suspension of human mitochondria at the one- or two-cell stage. Human mtDNA was detected by PCR with species-specific primers. The total amount of the- and four-cell murine embryos analyzed in the study was 315. In all embryos examined together with murine mtDNA copies of human mitochondrial genome were revealed indicating the phenomenon of an artificially modeled heteroplasmy. Foreign mtDNA was not ubiquitous in blastomeres of transmitochondrial embryos. Mathematical treatment of the results showed that, in the period between the injection of human mitochondria and the subsequent embryo cleavage, an uneven distribution of human mtDNA occurred in the cytoplasm. These results also indicate the presence of more than two to three segregation units of mtDNA in the entire pool of mitochondria (about 500) introduced into an embryo by microinjection.
Databáze: OpenAIRE
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