| Popis: |
The aim of the work is to show the ecological, epizootological and epidemiological significance of ixodic ticks in the distribution and circulation of tick-borne pathogens in the south of the Far East. Methods. The material for the study was collected during an expedition conducted in the spring and summer of 2018 on five routes: in the northwestern territories of the Primorsky Territory (Spassky District, Lesozavodsky City District) and in the northern territories (Krasnoarmeysky District). 830 copies of ixodic ticks were collected. 187 samples were studied using real-time polymerase chain reaction (PCR-RT). Results. Of the total number of ticks collected, route 1 accounted for 24.7 %, 2 - 17.4 %, 3 - 19.5 %, 4 - 23.0 %, 5 - 14.8 %. It was shown that ticks I. persulcatus (73.1 ± 4.3) - (95.9 ± 1.5) % were the dominant species on all studied routes except route 3. On all routes, tick infection by I. persulcatus was most often observed, in which RNA tick-borne encephalitis virus was detected in 2 cases (1.9 %), DNA B. burgdorferi s. l. - in 40 (37.7 %), DNA B. miyamotoi - in 1 (0.9 %), DNA A. phagocytophilum - in 7 (6.6 %), DNA E. chaffeensis / E. muris-FL - 1 (0.9 %). In ticks of the Haemaphysalis genus, pathogens were detected only in H. japonica: - in 1 pool (1.2 %), DNA of R. heilongjiangensis - in 3 (3.7 %). Besides in 6 pools of ticks I. persulcatus, mixes of genetic markers of DNA of B. burgdorferi s. l. with various pathogens were detected. Conclusions. The data obtained indicate a different degree of infection of ixodic ticks with tick-borne pathogens, which indicates the need for monitoring studies with a wide coverage of all territories in the south of the Far East. |
