Properties of Hydrangea Ringspot Virus Ribonucleic Acid

Autor:	R. J. Zeyen, John H. Hill, Helen I. Benner
Rok vydání:	1977
Předmět:	Hydrangea ringspot virus biology Polyacrylamide Formaldehyde Hyperchromicity RNA biology.organism_classification Virology Electrophoresis chemistry.chemical_compound chemistry Nucleic acid Denaturation (biochemistry)
Zdroj:	Journal of General Virology. 34:115-125
ISSN:	1465-2099 0022-1317
DOI:	10.1099/0022-1317-34-1-115
Popis:	Summary Hydrangea ringspot virus has been purified to near homogeneity. Virus nucleic acid was identified as single-stranded RNA with an S value of 33·6 before and 21·1 after formaldehyde denaturation. Nucleic acid mol. wt. determinations made by linear log sucrose density gradient centrifugation gave values of 2·25 × 106 and 2·14 × 106, respectively, for native and formaldehyde-treated RNA. Electrophoresis on polyacrylamide gels before and after formaldehyde denaturation gave mol. wt. values of 2·64 × 106 and 2·03 × 106. The values from formaldehyde denatured RNAs are considered the most reliable. Thermal denaturation profiles showed a Tm of 55·2 °C in 0·1 m-sodium phosphate, pH 7·0, and a hyperchromicity of 22·4%.
Databáze:	OpenAIRE
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