Anti-proliferative and Anti-metastatic Potential of High Molecular Weight Secretory Molecules from Probiotic Lactobacillus Reuteri Cell-Free Supernatant Against Human Colon Cancer Stem-Like Cells (HT29-ShE)
| Autor: | Hamid Madanchi, Mehdi Kadivar, Mahdi Rohani, Zohreh Saltanatpour, Behrooz Johari, Faezeh Maghsood |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
chemistry.chemical_classification
education.field_of_study biology 010405 organic chemistry Chemistry Population Bioengineering biology.organism_classification Polysaccharide 01 natural sciences Biochemistry Molecular medicine Molecular biology 0104 chemical sciences Analytical Chemistry law.invention Lactobacillus reuteri Probiotic law In vivo Apoptosis Cancer stem cell Drug Discovery Molecular Medicine education |
| Zdroj: | International Journal of Peptide Research and Therapeutics. 26:2619-2631 |
| ISSN: | 1573-3904 1573-3149 |
| DOI: | 10.1007/s10989-020-10049-z |
| Popis: | Cancer stem cells population are necessary for cancerous invasion. Previous studies have indicated anti-tumor properties of probiotic Lactobacillus reuteri. At the current study, the effect of heat-killed sonicated (HK-SON) fraction and defined molecular weight secreted fractions from L. reuteri cell-free supernatant (CFS) on invasion and apoptosis levels of HT29-ShE cells was investigated. HT29-ShE cells were treated with a range of defined molecular weight fractions derived from CFS and HK-SON. A group of the cells was treated with uninoculated sterile bacterial growth media (MRS) as the negative control for 24 h. Afterward, real-time PCR analysis was done for investigating the relative production level of MMP-9, TIMP-1, and COX-2 mRNA. Matrigel-coated insert plates were used to evaluate cell invasion. The pro-apoptotic effect of CFS and HK-SON was determined using flowcytometry, and the gelatinolytic role of MMP-9 was analyzed via gelatin zymography. HK-SON treatment and defined fractions of CFS significantly decreased the cell invasion versus the MRS group (p 100 kD, and HK-SON fractions (p 100 kD fraction significantly up-regulated the production level of TIMP-1 (p 100 kD fractions significantly increased the apoptosis percentage of HT29-ShE cells. Our results indicate the anti-metastatic and anti-proliferative properties of CFS derived from L. reuteri. Findings suggest that a secretory macromolecule (s) such as a polysaccharide, nucleic acid, or protein might exert these inhibitory effects on colon cancer stem-like cells. In conclusion, our results indicated that cell-free supernatant components can be used as the anti-metastatic agent. However, this study must be performed in experimental and in vivo models for further results. |
| Databáze: | OpenAIRE |
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