CD103+ T and Dendritic Cells Indicate a Favorable Prognosis in Oral Cancer
| Autor: | L Q Fu, Hao Li, Qi-Chao Yang, Liang Mao, Yao Xiao, Bing Liu, Z.J. Sun, Cong-Cong Wu |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Tumor microenvironment Stromal cell medicine.diagnostic_test Chemistry medicine.medical_treatment CD11c Cancer hemic and immune systems chemical and pharmacologic phenomena Immunotherapy medicine.disease Flow cytometry 03 medical and health sciences 030104 developmental biology 0302 clinical medicine 030220 oncology & carcinogenesis medicine Cancer research Immunohistochemistry General Dentistry CD8 |
| Zdroj: | Journal of Dental Research. 98:1480-1487 |
| ISSN: | 1544-0591 0022-0345 |
| DOI: | 10.1177/0022034519882618 |
| Popis: | T cells and dendritic cells (DCs) that are positive for the tissue-resident marker CD103 play a vital role in antitumor immunity. In this study, multiplexed immunohistochemistry was applied to stain CD103 and the T-cell marker CD8 as well as the DC marker CD11c on formalin-fixed, paraffin-embedded oral squamous cell carcinoma (OSCC) tissues. Then, the density of CD103+CD8+ and CD103+CD11c+ tumor-infiltrating lymphocytes (TILs) in the intratumoral and stromal regions was calculated, and the correlation of CD103+CD8+ TIL and CD103+CD11c+ TIL density with OSCC patient prognosis was analyzed. The results revealed that CD103+CD8+ TILs and CD103+CD11c+ TILs were abundant in the stromal region and that increased stromal CD103+CD8+ TIL and intratumoral CD103+CD11c+ TIL density indicated a favorable prognosis. Moreover, we freshly isolated TILs from OSCC samples and performed flow cytometry to verify that CD103+CD8+ TILs display a tissue-resident memory T-cell (Trm) phenotype, and we discriminated CD103+CD11c+ TILs from tumor-associated macrophages. |
| Databáze: | OpenAIRE |
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