Bioconversion of Colloidal Chitin Using Novel Chitinase from Glutamicibacter uratoxydans Exhibiting Anti-fungal Potential by Hydrolyzing Chitin Within Fungal Cell Wall
| Autor: | Syeda Bushra Zafar, Shah Ali Ul Qader, Asma Ansari, Afsheen Aman, Tayyaba Asif, Urooj Javed |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
chemistry.chemical_classification Environmental Engineering biology Hypha Renewable Energy Sustainability and the Environment Bioconversion 020209 energy 02 engineering and technology biology.organism_classification 01 natural sciences Aspergillus fumigatus Cell wall chemistry.chemical_compound Enzyme Chitin chemistry Biochemistry 010608 biotechnology Hydrolase Chitinase 0202 electrical engineering electronic engineering information engineering biology.protein Waste Management and Disposal |
| Zdroj: | Waste and Biomass Valorization. 11:4129-4143 |
| ISSN: | 1877-265X 1877-2641 |
| Popis: | Chitin is a unique structural exopolysaccharide abundantly found in nature. This exopolysaccharide has a unique chemical structure that acts as a protective outermost covering for most of the crustaceans in aquatic ecosystem. This fortification is because of the insoluble nature of this exopolysaccharide which consist of a linear chain of β-(1→4)-linked-N-acetylglucosamine units. Chitin is hydrolyzed with the help of a hydrolase known as chitinase. Variety of microbial species have been explored for chitinase production. Chitinolytic microbial species can be alternatively used for degradation of chitin instead of chemical treatment in agricultural sector. This biological approach has lesser environmental impact because of its apparently safe nature. In the current study, bioprospecting of chitinase producing species was conducted and different chitinolytic bacterial strains were screened for chitinase production which could have anti-fungal potential. Bacterial isolates were identified based on polyphasic approach and the enzyme production was optimized using one-variable-at-a-time technique. Hyphal extension method was used for determination of anti-fungal potential of chitinase.Glutamicibacter uratoxydans was indigenously isolated and identified for chitinase production. G. uratoxydans is a novel bacterial species which has not been previously explored to produce chitinase or other hydrolases. G. uratoxydans biosynthesized chitinase utilizing colloidal chitin as a sole source of carbon. The chitinase biosynthesized by G. uratoxydans is effectively potent against Aspergillus fumigatus thus, suggesting that this extracellular enzyme could be used for the treatment of fungal infection caused by filamentous fungi. |
| Databáze: | OpenAIRE |
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