Enzyme-catalyzed simultaneous hydrolysis-glycolysis reactions reveals tunability on PET depolymerization products
| Autor: | Hercilio de Angeli Honorato, Luiz Silvino Chinelatto Júnior, Sonia Maria Cabral de Menezes, Lys Sirelli, Adriano Carniel, Marcos L. Dias, Aline Machado de Castro |
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| Rok vydání: | 2018 |
| Předmět: |
Cutinase
Environmental Engineering biology Chemistry Depolymerization Biomedical Engineering Bioengineering Context (language use) 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology biology.organism_classification 01 natural sciences 0104 chemical sciences Enzyme catalysis Catalysis Hydrolysis chemistry.chemical_compound Organic chemistry Candida antarctica 0210 nano-technology Ethylene glycol Biotechnology |
| Zdroj: | Biochemical Engineering Journal. 137:239-246 |
| ISSN: | 1369-703X |
| DOI: | 10.1016/j.bej.2018.06.007 |
| Popis: | PET depolymerization via enzymatic catalysis is one of the most promising technologies in the context of plastics circular economy. So far, hydrolysis reactions accounted for almost the totality of studies, since the glycolysis route was only recently reported. In the present work, a comparative analysis of hydrolysis and glycolysis of PET oligomers catalyzed by Humicola insolens cutinase (HiC) and Candida antarctica lipase B (CALB) showed completely distinct profiles of the reaction products and rates. Then, simultaneous hydrolysis-glycolysis reactions with varied water and ethylene glycol concentrations revealed a gradual increase of the esterified products mono(2-hydroxyethyl) terephthalate (MHET) and bis(2-hydroxyethyl) terephthalate (BHET) as the reaction medium became more organic. Erosion patterns on the PET surface during hydrolysis and glycolysis reactions were also shown to be different. The results here reported open new opportunities for the use of task-specific solvents for biotechnological PET recycling, providing versatility for the depolymerization products in different reuse technologies. |
| Databáze: | OpenAIRE |
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