Diversity and community structure of wood-inhabiting fungi found in Japanese wooden houses analyzed by the next-generation sequencing
| Autor: | Sakae Horisawa, Kiwamu Umezawa, Hisashi Abe, Tomoko Wada, Shuichi Doi, Makoto Yoshida, Ikuo Momohara, Masahiro Samejima |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
biology technology industry and agriculture Community structure Fungus biology.organism_classification complex mixtures DNA sequencing law.invention Biomaterials Cell wall 03 medical and health sciences 030104 developmental biology law Botany Internal transcribed spacer Mortierellales Ribosomal DNA Polymerase chain reaction |
| Zdroj: | Journal of Wood Science. 63:369-378 |
| ISSN: | 1611-4663 1435-0211 |
| DOI: | 10.1007/s10086-017-1630-7 |
| Popis: | The diversity and community structures of wood-inhabiting fungi in 16 decayed wood samples from ten wooden houses in Japan were analyzed using a next-generation sequencing (NGS) to determine the fungi responsible for wood decay. DNA of fungi in decayed wood was extracted directly, the internal transcribed spacer (ITS) region in ribosomal DNA (rDNA) was amplified by polymerase chain reaction (PCR), and then, sequences of tagged ITS fragments were analyzed by NGS. Results of sequencing indicated that 68 species of ascomycetes, 37 species of basidiomycetes, and one fungus each from Mortierellales and Mucoromycetes were detected. The fungal community structures showed diversity and included various species of ascomycetes. A microscopic examination of cell wall structure in decayed wood samples suggested that some ascomycetes were soft-rot fungi. Heat map analysis indicated that the similarity in the structures of fungal communities was influenced to a greater extent by the wood species of samples than where they were used as a component. |
| Databáze: | OpenAIRE |
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