Identification and determination of antibacterial constituents in residue discharged from garlic-processing plant
Autor: | Masato Nakamoto, Kayo Kunimura, Hirotaka Amano, Kensuke Ohishi, Junichiro Wakamatsu |
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Rok vydání: | 2020 |
Předmět: |
030309 nutrition & dietetics
Fractionation medicine.disease_cause Biochemistry Industrial and Manufacturing Engineering 03 medical and health sciences Residue (chemistry) 0404 agricultural biotechnology Levofloxacin medicine Food science 0303 health sciences biology Chemistry In vitro toxicology Biofilm 04 agricultural and veterinary sciences General Chemistry biochemical phenomena metabolism and nutrition Antimicrobial biology.organism_classification 040401 food science Staphylococcus aureus Bacteria Food Science Biotechnology medicine.drug |
Zdroj: | European Food Research and Technology. 246:1041-1049 |
ISSN: | 1438-2385 1438-2377 |
Popis: | Activity-guided fractionation based on in vitro assays was used to identify antibacterial components in the residue wasted in the manufacturing process of aged garlic extract (AGE). Antimicrobial activity of the hexane extract of aged garlic-derived residue (AGR) was evaluated against clinically isolated Gram-positive and -negative bacteria including levofloxacin (LVX)-resistant strains. Among antimicrobial fractions separated from the hexane extract of AGR, several polysulfides, such as diallyl-tetrasulfide (DAS4), -pentasulfide (DAS5) and -hexasulfide (DAS6), were identified as active components, whose chemical structures were determined by means of LC–MS and NMR spectroscopy. We found that those sulfides exhibited antimicrobial activities against Gram-positive bacteria at minimum inhibitory concentrations (MICs) ranging from 32 to 80 μg/mL. Although these sulfides were less active than LVX against most of the bacteria examined, they were more potent against Staphylococcus aureus (S. aureus) including methicillin-resistant S. aureus (MRSA). Surprisingly, DAS5 significantly suppressed the biofilm formation of S. aureus by 60% at a concentration of 1.25 μg/mL, which is extremely lower than its MIC value. These results show that AGR contains several antimicrobial diallyl-polysulfides which have the potential to inhibit the growth and biofilm formation of bacteria and AGR may be a useful bioresource as a natural additive for antibiotic drugs or foods. |
Databáze: | OpenAIRE |
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