Rapid sensing of antioxidant capacity based on electrochemiluminescence induced by electrochemically generated reactive oxygen species
| Autor: | Akiko Araki, Eiichi Tamiya, Yutaka Ogidou, Hiromi Ushijima, Masato Saito, Yuki Inoue, Naoki Nagatani, Yasuhiro Sakurai, Gen Hattori |
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| Rok vydání: | 2016 |
| Předmět: |
Detection limit
chemistry.chemical_classification endocrine system Reactive oxygen species Antioxidant Chemistry General Chemical Engineering Radical medicine.medical_treatment 010401 analytical chemistry 010402 general chemistry Photochemistry 01 natural sciences 0104 chemical sciences Luminol Standard curve chemistry.chemical_compound Electrochemistry medicine Electrochemiluminescence Trolox Nuclear chemistry |
| Zdroj: | Electrochimica Acta. 222:580-586 |
| ISSN: | 0013-4686 |
| Popis: | A simple and rapid electrochemiluminescence (ECL) method for the measurement of antioxidant capacity using a portable ECL device with a screen-printed electrode is described. The ECL method is based on the luminous reaction of reactive oxygen species (ROS), luminol radicals and antioxidants. ROS are generated by the electrochemical reduction of dissolved oxygen and competitively react with the luminol and antioxidants. The ECL intensity depends on the antioxidant capacity because the radicals are neutralized by the antioxidants, suppressing the luminous reaction. An antioxidant standard curve was generated using different concentrations of known amounts of Trolox by ECL measurement and the detection limit for antioxidant capacity was found to be 0.06 mM of Trolox. An antioxidant capacity of beverages (22 types) was evaluated by comparing with the standard curve of Trolox. The time necessary for the ECL measurement of antioxidant capacity is only two minutes after combining the sample solution with luminol on a screen-printed electrode. |
| Databáze: | OpenAIRE |
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