P488Lack of association of galectin-3 with myocardial fibrosis in patients with chronic stable heart failure of hypertensive origin
| Autor: | A. González Miqueo, R. Querejeta Iraola, B. López Salazar, J. Díez Martínez, M Larman Tellechea |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_specialty
Aldosterone Physiology business.industry Radioimmunoassay medicine.disease Brain natriuretic peptide chemistry.chemical_compound Endocrinology chemistry Galectin-3 Physiology (medical) Diabetes mellitus Heart failure Internal medicine medicine Myocardial fibrosis Cardiology and Cardiovascular Medicine business Coronary sinus |
| Zdroj: | Cardiovascular Research. 103:S89.2-S89 |
| ISSN: | 1755-3245 0008-6363 |
| DOI: | 10.1093/cvr/cvu091.163 |
| Popis: | Purpose: It has been proposed that galectin-3 (Gal-3) may be a pathogenic factor and a biomarker of myocardial fibrosis in patients with heart failure (HF). The aim of this study was to analyze whether Gal-3 is associated with myocardial fibrosis in patients with chronic stable HF. Methods: Endomyocardial biopsies and blood samples from non-diabetic, hypertensive patients without ischemic heart disease and with HF (n=39), and necropsies and blood samples from 7 and 20 control subjects, respectively, were studied. Left ventricular (LV) morphology and function were assessed by echocardiography. The area of the myocardium occupied by total collagen (CVF) was assessed by image analysis in Picrosirius Red-stained sections. Collagen type I and III CVFs were analyzed in sections immunostained for collagen type I or III. Gal-3 and collagen type I and III mRNA and protein expression were quantified by real time RT-PCR and western blot, respectively. Plasma Gal-3 and amino-terminal propeptide of brain natriuretic peptide (NT-proBNP) were determined by ELISA. Plasma aldosterone was measured by radioimmunoassay. Results: Myocardial Gal-3 protein (P |
| Databáze: | OpenAIRE |
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